Composite

Part:BBa_K2323002:Design

Designed by: Jorge Andres Quintero Serna   Group: iGEM17_Munich   (2017-10-25)


TEV protease with N-terminal 6x His-Tag under the control of the pT7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 71
    Illegal AgeI site found at 803
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite includes the 6xHis-Tag to allow purification for in-vitro use of the protein.


Source

The TEV protease sequence as well as the terminators and promoters come from BBa_K1319008.

References